This chapel is dedicated to St James, the patron saint of Spain. The altar piece, by Mattia Preti, depicts St James wearing a scallop shell on his chest, an attribute of pilgrims who visit his shrine. There are two grand masters buried in this chapel, the Portuguese nobleman Grand Master Anton Manoel de Vilhena (who reigned from 1722 to 1736) and Grand Master Pinto (who reigned from 1741 to 1773).